Flow Cytometry

Flow cytometry technology can be used to measure intrinsic cell characteristics such granularity, autofluorescence etc. and extrinsic cell characteristics such as DNA, internal and external receptors. This has many applications in humans, animals, plants and microorganisms with the most common applications in cell cycle, apoptosis/necrosis, ploidy determination, immunophenotyping, protein expression and Ca2+  concentration.

Instrumentation

Beckman Coulter CytoFLEX LX
The benchtop analyzer allows the analysis of blood or cultured cells, bacteria, algae, yeasts, microparticles and even nanoparticles like extracellular vesicles. 

CytoFLEX LX Platform flow cytometers demonstrates outstanding sensitivity and reproducibility with six excitation wavelengths  (375 nm, 405 nm, 488 nm, 561nm, 638 nm, 808 nm), able to collect 21 fluorescent markers, plus FSC and SSC. Scatter of Violet and Red laser are also available, as well as extra sets of filters. The CytoFLEX LX is equipped with a 96 well plate loader station.

This cytometer has the ability to reliably detect 80 nm polystyrene beads, the CytoFLEX is a trustworthy partner in your extracellular vesicle and other small particle research.

Beckman Coulter CytoFLEX S
This benchtop analyzer allows the analysis of blood or cultured cells, bacteria, algae, yeasts, microparticles and even nanoparticles like extracellular vesicles.

CytoFLEX Platform flow cytometers demonstrates outstanding sensitivity and reproducibility with four excitation wavelengths  (405 nm, 488 nm, 561nm, 638 nm), able to collect 13 fluorescent markers, plus FSC and SSC. Scatter of Violet and Red laser are also available, as well as extra sets of filters. The CytoFLEX S is equipped with a 96 well plate loader station.

This cytometer has the ability to reliably detect 80 nm polystyrene beads, the CytoFLEX is a trustworthy partner in your extracellular vesicle and other small particle research.

Expertise & Services

The flow cytometry facility provides a range of services including instrumentation, workstations, training, software for analyses as well as technical expertise and advice. Trained users prepare and run their own samples. Untrained users may avail of the sample running service and results analyses.

Bookings

Core technology bookings are made using an online portal, Bookkit by ClusterMarket. 

Existing user?
Login to the new Bookkit system 

First time user?
Contact the relevant staff member in the facility to register initially.

Contact:

• Assoc. Prof. Alfonso Blanco, Director, Flow Cytometry Core
• T: (+353-1) 716 6836 / 6947
• E: alfonso.blanco@ucd.ie