Multiplexed Assay Platforms

Multiplexing or multiple analyte detection offers a broad picture of the cytokines involved in a certain biological process. The measurement of cytokines and other soluble factors is becoming increasingly important in the study and management of numerous diseases.

Instrumentation

Luminex xMAP200 &  MagPix
The bead-based assays follow the same principle as a sandwich immunoassay. Fluorescent beads are coated with antigen-specific antibodies. A mixture of coated beads is incubated with the sample. Analytes in the sample bind to the Ab coating the beads. A biotin-conjugated Ab mix is added, which binds to the analytes bound to the capture Ab. A fluorochrome binds the biotin conjugates. Our instruments differentiates the bead populations and calculates the analyte concentration in the samples being analysed

Providing a rapid, reliable, cost-effective and informative solution, the main benefits of multiple analyte detection are:

• Reduced cost and labour by multiplexing
• Requires less than 50 µl of sample
• Shortened time-to-results by favourable reaction kinetics
• Give faster, more reproducible results than ELISA
• Flexible multiplexing in the range of 1 to 100 analytes meets the needs of a wide variety of applications such as protein expression profiling, focused gene expression profiling, autoimmune disease, genetic disease, molecular infectious disease, and HLA testing

Expertise & Services

The flow cytometry facility provides a range of services including instrumentation, workstations, training, software for analyses as well as technical expertise and advice. Trained users prepare and run their own samples. Untrained users may avail of the sample running service and results analyses.

Bookings

Core technology bookings are made using an online portal, Bookkit by ClusterMarket. 

Existing user?
Login to the new Bookkit system 

First time user?
Contact the relevant staff member in the facility to register initially.

Contact:

• Assoc. Prof. Alfonso Blanco, Director, Flow Cytometry Core Technology
• T: (+353-1) 716 6836 / 6947
• E: alfonso.blanco@ucd.ie