UCD Conway Institute of Biomolecular and Biomedical Research Institiúid Mhic Con Mí an Taighde Bhithmhóilínigh agus Bhithleighis

748w_MassSpec_Res_CT

Proteomics Core - Mass Spectrometry Resource

The UCD Conway Proteomics Core - Mass Spectrometry Resource facilitates study of biomolecules that play important roles in fundamental and applied biology, particularly in health and disease. Our instruments enable use of state of the art proteomics and metabolomics approaches to address your scientific problem, including quantitative proteomics, post-translational modifications, and protein-protein interactions.

We work with academic, clinical, and industry partners; from novices to experienced researchers. Our team of experts are ready to listen to your research needs and offer dedicated strategic support at all stages of your project. Contact us using the details listed in the Team & Contact Details section below.

 

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We use two mass spectometry platforms:

  • Thermo Scientific Orbitrap Exploris 480 with Vanquish Neo capillary chromatography system. This is a hybrid ESI-LCMS instrument that combines precursor ion selection using a quadrupole with high resolution high mass accuracy (HRAM) by Orbitrap
  • Bruker tims TOF Pro mass spectrometer with Evosep One chromatography system or Bruker nanoElute chromatography system. A trapped ion mobility (TIMS) analyzer can be synchronized with a quadrupole mass filter to enable the highly efficient PASEF (Parallel Accumulation Serial Fragmentation acquisition) procedure with acquisition rates of 100 Hz.

 

orbBruker TimsTOF Pro

Access to both these instruments means that we can customise to your mass spectometry analysis needs, depending on sample complexity, target abundance or analytical strategy.

Protocols 

• Sample preparation for LC-MS/MS-Guidelines
• Protocol for in-gel Trypsin Digest

• Protocol for in-solution Trypsin digest (generic)
• Protocol for Filter Aided Sample Preparation (FASP)

• Sample Preparation: STAGE TIP clean up
• Protocol for Zip-Tipping tryptic digests

Software

We can help guide you to the various stages of experiment analysis, including Protein Identification and Quantitation, statistical evaluation, data mining, and pathway analysis.

Specialist analysis pipelines address applications including protein modification (PTM) analysis, de novo peptide sequencing, expresion proteomics, data-independent and data-dependent analysis (DIA, DDA). We use multicore processes operating on both Windows and Linux systems. 

MaxQuant PEAKS Mascot Perseus Cytoscape Skyline

Statment on files

Raw file sizes from mass spectometry experiments are very large and we have limited storage capacity. In general, we search you raw data (MS and MS/MS spectra) using appropriate software and provide you with the results (normally a spreadsheet of protein identities and relative abundances). However, after 12 months we will delete both sets of files. We will advise you on how to store and interpret your files, but it the user's responsibility to ensure that they collect or download their own files before this date.

Matthias Wilm

Prof. Matthias Wilm has extensive experience of mass spectrometry and proteomics. He contributed decisively to the development of proteomic techniques while he was at the European Molecular Biology Laboratory (EMBL).

Contact: matthias.wilm@ucd.ie

Eugene Dillon

Eugene studied nutritional sciences in University College Cork before specialising in molecular nutrition during his MSc at Wageningen University. Eugene obtained an IRCSET Phd scholarship to work with Prof. Helen Roche and Dr Giuliano Elia utilising proteomics in the study of nutritional metabolism. After his PhD, Eugene worked as a postdoctoral scientist with Food for Health Ireland. Over the course of his doctoral and postdoctoral studies, Eugene has amassed expertise across sample preparation, mass spectrometric methodology, data processing and downstream pathway analysis.

Contact: T: (+353-1) 716 6835; E: eugene.dillon@ucd.ie